Journal: Bioengineering

Article Title: Temporal Tissue Remodeling in Volumetric Muscle Injury with Endothelial Cell-Laden Patterned Nanofibrillar Constructs

doi: 10.3390/bioengineering11121269

Figure Lengend Snippet: Timeline of vascularization following VML injury and treatment with patterned EC-laden constructs. ( a ) Representative confocal microscopy images of transverse cross-sections of TA muscle depicting isolectin-positive blood vessels (red) with mask overlays of individually segmented vessels generated by Cellpose on days 2, 7, and 21 post injury; ( b , c ) Quantification of vascular density ( b ) and vessel-to-myofiber ratio ( c ) within a 500 µm area from an Acellular Aligned, EC Random, or EC Aligned construct on days 2, 7, and 21 post injury (N = 4). Scale bars: ( a ) 50 µm. Significance was determined using a two-way ANOVA with p < 0.05 (*), p < 0.001 (***), and p < 0.0001 (****). Values shown are mean ± SD.

Article Snippet: On days 2, 7, and 21 post VML injury, mice were injected via the tail vein with 200 µL of isolectin (GS-IB4 from Griffonia Simpicifolia, Alexa Fluor 647 conjugate, 100 µg/mL, Invitrogen, I32450), a fluorescently labeled endothelial binding protein that was used to identify perfused blood vessels.

Techniques: Construct, Confocal Microscopy, Generated

Journal: Genes

Article Title: Kir4.1 and Aqp4 Contribution to Schisis Cystic Water Accumulation and Clearance in the Rs1 Exon-1 Del XLRS Rat Model

doi: 10.3390/genes15121583

Figure Lengend Snippet: Primary antibodies with appropriate dilution used in fluorescent immunohistology.

Article Snippet: Isolectin B, GS-IB4, AF-647-conjugated , , Invitrogen , I32450 , 1:500.

Techniques:

Journal: Genes

Article Title: Kir4.1 and Aqp4 Contribution to Schisis Cystic Water Accumulation and Clearance in the Rs1 Exon-1 Del XLRS Rat Model

doi: 10.3390/genes15121583

Figure Lengend Snippet: Colocalization of Aqp4 expression with perivascular vessels at P30 in WT and Rs1KO rat retinas. The blood vessels (Isolectin B GS-IB4-silver) and Aqp4 (red) are expressed in MC tightly around blood vessels in WT, but in Rs1KO rat retinas, the expression is much more distributed. Confocal images show Aqp4 (in red), the endothelial cell marker Isolectin B, GS-IB4 (silver), and the nuclear marker DAPI (blue). Aqp4 was concentrated in the perivascular (arrows) and inner limiting membrane domains in the WT animals (scale bar = 50 µ).

Article Snippet: Isolectin B, GS-IB4, AF-647-conjugated , , Invitrogen , I32450 , 1:500.

Techniques: Expressing, Marker, Membrane

Journal: Genes

Article Title: Kir4.1 and Aqp4 Contribution to Schisis Cystic Water Accumulation and Clearance in the Rs1 Exon-1 Del XLRS Rat Model

doi: 10.3390/genes15121583

Figure Lengend Snippet: Disruption and thinning of the deep capillary plexus. ( a ) Retinal flatmounts of WT and Rs1KO rats at P30, with blood vessels labeled for isolectin B (GS-IB4 antibody, silver). ( b ) Enlarged regions of retinal flatmounts of WT and Rs1KO rats at P30, with blood vessels labeled for isolectin B (GS-IB4 antibody, silver). Aqp 4 (red) showed DCP disruption associated with MGC perivascular processes (scale bar = 60 µ).

Article Snippet: Isolectin B, GS-IB4, AF-647-conjugated , , Invitrogen , I32450 , 1:500.

Techniques: Disruption, Labeling